Bacteria, good and bad, is all around us. In this activity students will collect and grow bacteria and then learn techniques for studying the bacteria they have grown. They will perform a simple staining technique on bacterial cells to study the morphology. They will also perform a differential staining technique, Gram staining, on bacterial cells and determine if the collected bacteria are Gram-positive, Gram-negative, or both.
Aligned to the Next Generation Science Standards (NGSS)*
Disciplinary Core Ideas: LS1.A
Performance Expectations: MS-LS1-1; MS-LS1-2
Cross Cutting Concepts: Scale, Proportion and Quantity; Structure and Function
Engineering Practices: Developing and Using Models; Planning and Carrying out Investigations
20 Sterile Petri Dishes
2 x 200mL Prepared Nutrient Agar
20 Sterile Cotton Swabs, pk/2
1 x 30mL Methylene Blue
1 x 30mL Crystal Violet
1 x 30mL Gram s Iodine
1 x 30mL Safranin O
4 x 30mL Ethanol, 95%
1 pk/72 Microscope Slides
1 pk/100 Coverslips
Small quantity exemption 173.4
THIS PACKAGE CONFORMS TO 49 CFR 173.4 for domestic highway or rail transport only
* Next Generation Science Standards is a registered trademark of Achieve. Neither Achieve nor the lead states and partners that developed the Next Generation Science Standards was involved in the production of, and does not endorse, this product.
WARNING: This product can expose you to chemicals including Crystal Violet and Methanol/Methyl isobutyl ketone, which are known to the State of California to cause cancer and reproductive harm. For more information go to http://www.P65Warnings.ca.gov.