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Teach your students about synthetic biology with this exciting and exclusive lab! Students use PCR to amplify the coding sequence of the BSMT1 enzyme. This interesting enzyme is responsible for the formation of methyl salicylate, a In this lab activity, students will investigate synthetic biology using the PCR to discover the coding sequence of the BSTM1 enzyme. The PCR product provides a resulting recombinant DNA that is used to transform E. coli BactoBeads, allowing students to perform a smell test experiment ensuring the bacterial factories are working from the enzyme.
PCR EdvoBeadsPrimer mix concentrateTemplate DNA concentrateUniversal DNA bufferUltrapure waterDNA standard marker10X gel loading solutionPurification and wash buffersSpin columns and reservoirsDryzyme mixDryzyme reconstitution bufferEnzyme reaction buffervector cut with EcoRI and HindIIIT4 DNA ligaseReadyPour LB agarLB mediumBactoBeads (Host)Calcium ChlorideCompetent cell solutionControl DNARecovery brothKanamycinIPTGSalacylic acidwintergreen scent50X electrophoresis bufferUltraSpec-AgaroseSYBR Safe Stainmicrocentrifuge and PCR tubes10 ml pipet50 ml conical tubesSterile loops.